Friend or Foe: Transcription and RNA Meet DNA Replication and Repair

Event Report by Apoorva Baluapuri, University of Würzburg, Germany

RNA and DNA were first described by the Swiss biologist Friedrich Miescher in 1868. About 150 years later, we stand at crossroads of the two disciplines which have arisen as a result of dedicated research on both molecules. The first EMBL symposium on the connections between transcription and DNA replication/repair research was a major step forward in combining the progress from wide ranging topics, thus generating a consensus on how gene expression and DNA transactions cooperate.

The symposium, which was the second one from EMBL this year,  was scheduled just a day after International Women’s Day, and that aligned very well with the equally represented line-up of speakers and organisers!

The titular opening session was dedicated to transcription-associated genomic instability where all aspects of R-loops and ribonucleotide excision repair in transcription coupled DNA double strand break repair were covered. For example, Gaëlle Legube (CNRS – University of Toulouse, France) expanded in great detail on the influence of DSB-induced chromatin conformation and the strong potential of 3C-based technologies, while Elodie Hatchi (Dana Farber Cancer Institute, Boston, USA) explained about her recent publication in Nature concerning the impact of BRCA1, RAD52 and PALB2 on small RNA-driven DNA repair.

Eventually, we switched over to a more translational theme with Rushad Pavri (IMP, Vienna, Austria) who spoke about the relation between DNA replication timing and frequency of oncogenic translocations.

This time around, the poster presentation sessions were equally dynamic with topics being covered from role of RBMX in RNA processing (Sara Luzzi, University of Newcastle) to role of MYCN in reconciling elevated transcription levels with DNA replication (Dimitrios Papadopoulos, University of Würzburg, Germany).

To end the first day, Philippe Pasero (CNRS, France) tried to answer the old question of the chicken or the egg in terms of toxic R-loops, if they are the cause or consequences of DNA replication stress, while Andrew Deans (St. Vincent’s Institute of Medical Research, Australia) explained about fork re-modellers as a general mechanism of R-loop removal.

The second day started out on a high note by a talk on the consequences of DNA damage and heat shock on Pol II from Jesper Svejstrup. Prof Svejstrup recently moved his lab from the Francis Crick Institute in London to the University of Copenhagen (Faculty of Health and Medical Sciences).

To make things even more exciting at the symposium, Martin Eilers (University of Würzburg, Germany) spoke about conflict resolution by MYCN between “friends and foes”, i.e. Pol II and replication fork. This was followed by talk by Marco Foiani (University of Milan, Italy) who showed the role of ATR in nuclear integrity.

In between the breaks, the participants eagerly shared their setup of how they were joining the virtual conference:

Not only were the home setups on display, but we also got a glimpse of “add-on” participants at the conference:

Along with this fun, the second day’s poster session continued with equally interesting topics as the previous day. The virtual conference platform provided by Engagez came across as a handy tool in coming as close as possible to the in-person poster presentations.

Frédéric Chédin (University of California, Davis, USA) closed the day by talking about interplay between splicing and R-loops.

In the next two days, a wide variety of topics and methods were covered. For example,  Nick Proudfoot (University of Oxford, UK) dazzled with correlation between R-loops and antisense transcription while Petra Beli (IMB, Mainz, Germany) moved the focus from genomics to proteomics with èlan. She spoke about a method called “RDProx” which maps R-loop proximal proteome in a native chromatin environment.

Also, junior group leaders like Marco Saponaro (University of Birmingham, UK) answered what happens to replication when it encounters transcription and Madzia Crossley (Stanford University, USA) showed CytoDRIP-blots to probe RNA-DNA hybrids on gels which showed that SETX and BRCA1 loss, along with splicing inhibition, results accumulation of RNA-DNA hybrids in cytoplasm!

All along the talks, whichever questions (which, by the way, were in majority from younger researchers) didn’t get answered, were posted and responded to in the “Forum” section: this actually became a valuable summary of quite a few topics.

The networking options were also in abundance, be it the Virtual Bar mixer, or Meet the Editors session on the online platform. Given that editors from elite journals like EMBO, PLoS Biology etc. were present, it gave a nice opportunity for the researchers to gauge where their next big story could find a good home.

In summary, the symposium gave the feeling of being cozy without being too small and specific in terms of the topics covered, and benefited both the experienced and young researchers in an equal way. It was a common understanding and expectation among the participants that this symposium would perhaps be held in person next time if possible.

Follow us:

8 tips for preparing a digital poster that stands out from the crowd

Virtual meetings are rapidly gaining popularity, due largely to the necessity of continuing knowledge exchange during the social isolation brought on by the Corona pandemic.

Even before the pandemic, EMBL´s Course and Conference Office was already exploring options to improve our services and the event experience on-site, including the option of digital poster presentations.

Our software provider iPosterSessions comes with easy to use WYSIWYG templates. Users can display high-resolution images, videos & animations, and the content can be updated at any time right throughout the conference – allowing poster presenters to present their research digitally and dynamically.

If you are presenting a digital poster at an upcoming (virtual!) meeting, here are eight tips to help you on your way:

  1. Download the official template from the software provider

Most digital software providers have an official template that you can download – use it! This will reduce the risk of glitches, resolution problems and sizing issues in the final product, and you know from the outset what you have to work with.

  1. Check out the tutorials

No two digital poster tools are the same, so take the time to browse through the online tips and tutorials to make sure you are comfortable with the software before starting. It will save you a lot of frustration in the long run!

  1. Make your design eye-catching – it should stand out from the crowd

This is the same principle as creating a printed scientific poster – there are so many of them, so make sure yours stands out! It should be eye-catching and visually appealing. Include clear data representations, and make sure the text is to the point. It should grab attention but not explain every little thing about your results – that’s your job during the discussion.

  1. Use media – images, sounds, video. Check that they work and display properly

Graphics and media can express details more quickly and memorably than paragraphs of text, so have a think about how you can present your work in this way and put some time into it. Be sure to check that the media files work with the software, and test every file to make sure they display or play properly.

  1. Link to external resources

Digital posters differ from printed posters in that you can generally link to other pages online – so if there is a great external paper or online source you want to link to in order to explain your point in more detail, do it! Your audience will be grateful to have further reading handed to them on a plate if they want to find out more after the poster session.

  1. Check your work

This should really be a no-brainer. Check your work is complete, correct and final before publishing your poster! Silly mistakes only show that you haven’t put as much time and effort into the work as you probably should have, so get someone else to go over your poster before you release it to the conference community.

  1. Practice your presentation

Yes, it’s a digital poster presentation, and no, you won’t be talking face-to-face with your audience as you normally would, but you still need to practice your presentation beforehand and know exactly what you want to say and how you want to say it. It may feel strange online, so try presenting the poster online with a colleague or your boss (e.g. with Skype, Zoom, Google Hangouts) and get them to give you feedback and pointers.

  1. Stick to the publishing deadline

There are deadlines for a reason, so please stick to them! You don’t want to risk your poster being excluded from the poster presentation because of tardiness. Give yourself plenty of time in case of any issues that may arise with uploading or compatibility (this shouldn’t be an issue if you followed the template and guidelines, but sometimes computers have a mind of their own!).

So why not check out our list of upcoming virtual events to see where you can try out your digital poster presentation skills!

For general pointers about creating posters, see 10 tips to create a scientific poster people want to stop at.

Follow us: